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nf-core/evexplorer

nf-core/evexplorer is a pipeline for analyzing RNA data from extracellular vesicles, compatible with technologies such as nextflex, comboSeq, and ONT with further support forthcoming. evexplorer handles QC, expressed region detection, library size normalization and Differential RNA Expression (DRE)

Version: 0.0.0-c5677f2 Git revision: c5677f226d563a775179071105e92b7097e06c63 Commit: Content hash:
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Repository: nf-core/evexplorer
Workflow: main.nf

nf-core/evexplorer

GitHub Actions CI Status GitHub Actions Linting StatusAWS CICite with Zenodo nf-test

Nextflow run with conda run with docker run with singularity Launch on Seqera Platform

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Introduction

nf-core/evexplorer is a bioinformatics pipeline that ...

  1. Read QC (FastQC)
  2. Present QC for raw reads (MultiQC)

Usage

Note

If you are new to Nextflow and nf-core, please refer to this page on how to set-up Nextflow. Make sure to test your setup with -profile test before running the workflow on actual data.

Now, you can run the pipeline using:

nextflow run nf-core/evexplorer \
   -profile <docker/singularity/.../institute> \
   --input samplesheet.csv \
   --outdir <OUTDIR>

Warning

Please provide pipeline parameters via the CLI or Nextflow -params-file option. Custom config files including those provided by the -c Nextflow option can be used to provide any configuration except for parameters; see docs.

For more details and further functionality, please refer to the usage documentation and the parameter documentation.

Pipeline output

To see the results of an example test run with a full size dataset refer to the results tab on the nf-core website pipeline page. For more details about the output files and reports, please refer to the output documentation.

Credits

nf-core/evexplorer was originally written by .

We thank the following people for their extensive assistance in the development of this pipeline:

Contributions and Support

If you would like to contribute to this pipeline, please see the contributing guidelines.

For further information or help, don't hesitate to get in touch on the Slack #evexplorer channel (you can join with this invite).

Citations

An extensive list of references for the tools used by the pipeline can be found in the CITATIONS.md file.

You can cite the nf-core publication as follows:

The nf-core framework for community-curated bioinformatics pipelines.

Philip Ewels, Alexander Peltzer, Sven Fillinger, Harshil Patel, Johannes Alneberg, Andreas Wilm, Maxime Ulysse Garcia, Paolo Di Tommaso & Sven Nahnsen.

Nat Biotechnol. 2020 Feb 13. doi: 10.1038/s41587-020-0439-x.

32 parameter(s) in 5 group(s)

Generic options#

Less common options for the pipeline, typically set in a config file.

Display help text.

type boolean

Display version and exit.

type boolean

Incoming hook URL for messaging service

More detail

Incoming hook URL for messaging service. Currently, MS Teams and Slack are supported.

type string

Email address for completion summary, only when pipeline fails.

More detail

An email address to send a summary email to when the pipeline is completed - ONLY sent if the pipeline does not exit successfully.

type string
pattern ^([a-zA-Z0-9_\-\.]+)@([a-zA-Z0-9_\-\.]+)\.([a-zA-Z]{2,5})$

Custom config file to supply to MultiQC.

type string

Do not use coloured log outputs.

type boolean

Send plain-text email instead of HTML.

type boolean

Boolean whether to validate parameters against the schema at runtime

type boolean
default true

Method used to save pipeline results to output directory.