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nf-core/biscuit/biscuitblaster @ 0.0.0-6c4ed3a

A fast, compact one-liner to produce duplicate-marked, sorted, and indexed BAM files using Biscuit

Latest version: 0.0.0-6c4ed3a
Total downloads: 6
Source: nf-core/modules
Authors: @njspix
Maintainers: @njspix @sateeshperi

Summary

A fast, compact one-liner to produce duplicate-marked, sorted, and indexed BAM files using Biscuit

Get started

Add the following snippet to your workflow script to include this module.

include { BISCUIT_BLASTER } from 'nf-core/biscuit/biscuitblaster'

License

MIT License

Process
Name BISCUIT_BLASTER
Input 3 channels
#1 tuple
meta map

Groovy Map containing sample information e.g. [ id:'test', single_end:false ]

reads file

List of input FastQ files of size 1 and 2 for single-end and paired-end data, respectively.

#2 tuple
meta2 map

Groovy Map containing sample information e.g. [ id:'test', single_end:false ]

fasta file

Input genome fasta file

#3 tuple
meta3 map

Groovy Map containing sample information e.g. [ id:'test', single_end:false ]

index directory

Directory containing biscuit genome index

Output 5 channels
#1 bai tuple
meta map

Groovy Map containing sample information e.g. [ id:'test', single_end:false ]

*.bai file

Output BAM index

*.{bai}
#2 bam tuple
meta map

Groovy Map containing sample information e.g. [ id:'test', single_end:false ]

*.bam file

Output BAM file containing read alignments

*.{bam}
#3 versions_biscuit tuple
${task.process} string

The name of the process

biscuit string

The name of the tool

biscuit version |& sed '1!d; s/^.*BISCUIT Version: //' eval

The expression to obtain the version of the tool

#4 versions_samtools tuple
${task.process} string

The name of the process

samtools string

The name of the tool

samtools --version |& sed '1!d; s/^.*samtools //' eval

The expression to obtain the version of the tool

#5 versions_samblaster tuple
${task.process} string

The name of the process

samblaster string

The name of the tool

samblaster --version |& sed 's/^.*samblaster: Version //' eval

The expression to obtain the version of the tool

Tool Description Homepage
biscuit A utility for analyzing sodium bisulfite conversion-based DNA methylation/modification data https://huishenlab.github.io/biscuit/
samblaster samblaster is a fast and flexible program for marking duplicates in read-id grouped paired-end SAM files. It can also optionally output discordant read pairs and/or split read mappings to separate SAM files, and/or unmapped/clipped reads to a separate FASTQ file. By default, samblaster reads SAM input from stdin and writes SAM to stdout. n/a
samtools SAMtools is a set of utilities for interacting with and post-processing short DNA sequence read alignments in the SAM, BAM and CRAM formats, written by Heng Li. These files are generated as output by short read aligners like BWA. http://www.htslib.org/
Version 0.0.0-6c4ed3a
Commit ID 6c4ed3a220310b905a1fc9d04f05be2e0837142b
Release Date 23 Apr 2026 15:06:14 (UTC)
Download URL https://registry.nextflow.io/api/v1/modules/nf-core%2Fbiscuit%2Fbiscuitblaster/0.0.0-6c4ed3a/download