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nf-core/isoseq3/tag @ 0.0.0-6c4ed3a

Extract UMI and cell barcodes

Latest version: 0.0.0-6c4ed3a
Total downloads: 10
Source: nf-core/modules
Authors: @skraettli
Maintainers: @skraettli

Summary

Extract UMI and cell barcodes

Get started

Add the following snippet to your workflow script to include this module.

include { ISOSEQ3_TAG } from 'nf-core/isoseq3/tag'

License

MIT License

Process
Name ISOSEQ3_TAG
Input 2 channels
#1 tuple
meta map

Groovy Map containing sample information e.g. [ id:'sample1' ]

bam file

BAM file, one full-length CCS file generated by lima

*.5p--3p.bam
design string

Barcoding design. Specifies which bases to use as cell/molecular barcodes.

^(?:\d{1,2}[UBGX]-)+T$|^(?:\d{1,2}[UBGX]-)+T(?:-\d{1,2}[UBGX])+$|^T(?:-\d{1,2}[UBGX])+$
Output 3 channels
#1 bam tuple
meta map

Groovy Map containing sample information e.g. [ id:'sample1']

*.flt.bam file

BAM file with full-length tagged reads

*.flt.bam
#2 pbi tuple
meta map

Groovy Map containing sample information e.g. [ id:'sample1']

*.flt.bam.pbi file

Pacbio index file of full-length tagged reads

*.flt.bam.pbi
#3 versions
versions.yml file

File containing software versions

versions.yml
Tool Description Homepage
isoseq3 Iso-Seq - Scalable De Novo Isoform Discovery https://github.com/PacificBiosciences/IsoSeq/tree/master
Version 0.0.0-6c4ed3a
Commit ID 6c4ed3a220310b905a1fc9d04f05be2e0837142b
Release Date 23 Apr 2026 15:26:10 (UTC)
Download URL https://registry.nextflow.io/api/v1/modules/nf-core%2Fisoseq3%2Ftag/0.0.0-6c4ed3a/download
OCI Store URL https://public.cr.seqera.io/v2/nextflow/plugin/modules/nf-core/isoseq3/tag/blobs/sha256:2585342302f398af0b0a2678fa5ccd1f7133a976f9c60cdf483c75259bf440b4
Size 2.6 KB
Checksum sha256:2585342302f398af0b0a2678fa5ccd1f7133a976f9c60cdf483c75259bf440b4
Downloads 3
Version Date Status Downloads Size
0.0.0-6c4ed3a 23 Apr 2026 15:26:10 (UTC) 3 2.6 KB
0.0.0-3fc6d40 22 Apr 2026 15:18:13 (UTC) 3 2.6 KB
0.0.0-0c7146d 08 Apr 2026 19:11:11 (UTC) 4 2.5 KB