Nextflow Registry | nf-core/popscle/demuxlet@0.0.0-6c4ed3a

nf-core/popscle/demuxlet @ 0.0.0-6c4ed3a

Software to deconvolute sample identity and identify multiplets when multiple samples are pooled by barcoded single cell sequencing and external genotyping data for each sample is available.

popscle demultiplexing genotype-based deconvoltion single cell

Latest version: 0.0.0-6c4ed3a

Total downloads: 8

Source: nf-core/modules

Authors: @mari-ga @maxozo @wxicu @Zethson

Maintainers: @mari-ga @maxozo @wxicu @Zethson

Summary

Software to deconvolute sample identity and identify multiplets when multiple samples are pooled by barcoded single cell sequencing and external genotyping data for each sample is available.

Get started

Add the following snippet to your workflow script to include this module.

include { POPSCLE_DEMUXLET } from 'nf-core/popscle/demuxlet'

License

MIT License

Process

`Name`	`POPSCLE_DEMUXLET`

Input 1 channel

#1 tuple

`meta` map	Groovy Map containing sample information e.g. `[ id:'sample1']`
`plp_prefix` string	Prefix of pileup files (CEL,VAR and PLP) produced by popscle/dsc_pileup.
`bam` file	Input SAM/BAM/CRAM file without running popscle/dsc_pileup, must be sorted by coordinates and indexed. `*.{bam,cram,sam}`
`donor_genotype` file	Input VCF/BCF file, containing the individual genotypes (GT), posterior probability (GP), or genotype likelihood (PL) to assign each barcode to a specific sample (or a pair of samples) in the VCF file. `*.{vcf,bcf}`

Output 2 channels

#1 versions

`versions.yml` file	File containing software versions `versions.yml`

#2 demuxlet_result tuple

`meta` map	Groovy Map containing sample information e.g. `[ id:'sample1']`
`*.best` file	Result of demuxlet containing the best guess of the sample identity, with detailed statistics to reach to the best guess. `*.best`

Tool	Description	Homepage
popscle	A suite of population scale analysis tools for single-cell genomics data including implementation of Demuxlet / Freemuxlet methods and auxiliary tools	https://github.com/statgen/popscle

Version	0.0.0-6c4ed3a
Commit ID	6c4ed3a220310b905a1fc9d04f05be2e0837142b
Release Date	23 Apr 2026 15:38:12 (UTC)
Download URL	https://registry.nextflow.io/api/v1/modules/nf-core%2Fpopscle%2Fdemuxlet/0.0.0-6c4ed3a/download
OCI Store URL	https://public.cr.seqera.io/v2/nextflow/plugin/modules/nf-core/popscle/demuxlet/blobs/sha256:62961c0d1fc76e10a2a638530a2d34700579e8324ae900907f433593f0f81843
Size	2.9 KB
Checksum	sha256:62961c0d1fc76e10a2a638530a2d34700579e8324ae900907f433593f0f81843
Downloads	3

Version	Date	Downloads	Size
0.0.0-6c4ed3a	23 Apr 2026 15:38:12 (UTC)	3	2.9 KB
0.0.0-3fc6d40	22 Apr 2026 15:27:20 (UTC)	3	2.9 KB
0.0.0-0c7146d	08 Apr 2026 19:28:04 (UTC)	2	2.9 KB