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nf-core/rsem/calculateexpression @ 0.0.0-6c4ed3a

Calculate expression with RSEM

Latest version: 0.0.0-6c4ed3a
Total downloads: 6
Source: nf-core/modules
Maintainers: @drpatelh @kevinmenden

Summary

Calculate expression with RSEM

Get started

Add the following snippet to your workflow script to include this module.

include { RSEM_CALCULATEEXPRESSION } from 'nf-core/rsem/calculateexpression'

License

MIT License

Process
Name RSEM_CALCULATEEXPRESSION
Input 2 channels
#1 tuple
meta map

Groovy Map containing sample information e.g. [ id:'test', single_end:false ]

reads file

Input reads for quantification (FASTQ files or BAM file for --alignments mode)

*.{fastq.gz,bam}
index file

RSEM index

rsem/*
Output 9 channels
#1 logs tuple
meta map

Groovy Map containing sample information e.g. [ id:'sample1', single_end:false ]

*.log file

RSEM logs

*.log
#2 stat tuple
meta map

Groovy Map containing sample information e.g. [ id:'sample1', single_end:false ]

*.stat file

RSEM statistics

*.stat
#3 bam_star tuple
meta map

Groovy Map containing sample information e.g. [ id:'sample1', single_end:false ]

*.STAR.genome.bam file

BAM file generated by STAR (optional)

*.STAR.genome.bam
#4 bam_genome tuple
meta map

Groovy Map containing sample information e.g. [ id:'sample1', single_end:false ]

${prefix}.genome.bam file

Genome BAM file (optional)

*.genome.bam
#5 counts_gene tuple
meta map

Groovy Map containing sample information e.g. [ id:'sample1', single_end:false ]

*.genes.results file

Expression counts on gene level

*.genes.results
#6 versions_rsem tuple
${task.process} string

The name of the process

rsem string

The name of the tool

rsem-calculate-expression --version | sed 's/Current version: RSEM v//' eval

The expression to obtain the version of the tool

#7 versions_star tuple
${task.process} string

The name of the process

star string

The name of the tool

STAR --version | sed -e "s/STAR_//g" eval

The expression to obtain the version of the tool

#8 bam_transcript tuple
meta map

Groovy Map containing sample information e.g. [ id:'sample1', single_end:false ]

${prefix}.transcript.bam file

Transcript BAM file (optional)

*.transcript.bam
#9 counts_transcript tuple
meta map

Groovy Map containing sample information e.g. [ id:'sample1', single_end:false ]

*.isoforms.results file

Expression counts on transcript level

*.isoforms.results
Tool Description Homepage
rseqc RSEM: accurate transcript quantification from RNA-Seq data with or without a reference genome https://github.com/deweylab/RSEM
Version 0.0.0-6c4ed3a
Commit ID 6c4ed3a220310b905a1fc9d04f05be2e0837142b